Airborne fungi isolated from different environments of a primary school in the city of Manaus, Amazonas, Brazil / Fungos anemófilos isolados de diferentes ambientes de uma escola primária na cidade de Manaus, Amazonas, Brasil

Introduction: Airborne fungi can cause respiratory diseases, including pulmonary mycoses. The objective of this study was to isolate and identify airborne fungi from external and internal environments at a full-day primary school in Manaus, Brazil, and ascertain the influence of seasonality on the incidence of these microorganisms. Methods: Airborne fungi were collected by exposing Sabouraud agar plates at various external and internal locations in the school. Results: A total of 2,386 fungal colonies were isolated, 1,041 in the rainy season and 1,345 during the dry season. Of these, 1,858 were identified and distributed into 34 genera. The most prevalent were Cladosporium sp. (22.6%); Aspergillus sp. (17.14%); Penicillium sp. (8.55%); Curvularia sp. (6.83%); and Drechslera sp. (5.7%). During the dry season, the most prevalent genre was Aspergillus (19.3%), while in the rainy season, Cladosporium predominated (34.6%). Conclusion: Seasonality influenced fungal incidence, especially of the genus Cladosporium, which increased significantly during the rainy season. Cladosporium can be considered a bioindicator of the rainy season in the Brazilian Amazon.

Introdução: Os fungos presentes no ar, denominados anemófilos, possuem uma ampla diversidade em locais de clima tropical e são causadores de micoses pulmonares e outras doenças do aparelho respiratório. O objetivo do estudo foi isolar e identificar os fungos do ar de uma escola de ensino fundamental de tempo integral, a partir de ambientes externos e internos, e verificar se a sazonalidade influencia a incidência desses microrganismos. Métodos: Para coleta dos fungos do ar, placas de Petri contendo Sabouraud foram expostas nos ambientes externos e internos da escola. Resultados: Foram isoladas 2.386 colônias de fungos, sendo 1.041 na estação chuvosa e 1.345 na estação seca. Foram identificados 1.858 fungos, que puderam ser distribuídos em 34 gêneros. Os gêneros mais frequentes foram Cladosporium sp. (22,6%), Aspergillus sp. (17,14%), Penicillium sp. (8,55%), Curvularia sp. (6,83%) e Drechslera sp. (5,7%). Durante o período seco, o gênero mais frequente foi o Aspergillus (19,21%), e no período chuvoso, o gênero Cladosporium (34,8%). Conclusão: A sazonalidade influenciou principalmente o gênero Cladosporium, que obteve aumento significativo na estação chuvosa, constituindo um biomarcador dessa estação.

Biosurfactant Production by Fusarium oxysporum LM 5634 Using Peels from the fruit of Bactris gasipaes (Kunth) as Substrate

Aim: The aim of this work was to find new fungal sources of biosurfactants via bioprospecting and to produce biosurfactants from fungal isolates using inexpensive culture media. Methodology: Bioprocess tests were conducted with five fungal species isolated from soil from the Amazon region in order to investigate which of them presented the highest productivity in a kinetics experiment. The possibility of using the peels from the fruit of Astrocaryum aculeatum Meyer (PFAC), Bactris gasipaes Kunth (PFBG), Theobroma grandiflorum Schumann (PFTG), Musa paradisiaca (PFMP) as substrates was also evaluated. Results: Fusarium oxysporum LM 5634 had the highest productivity, and PFBG was the best substrate for biosurfactant production. Conclusion: Thus, the present study showed the potential of fungi and wastes (fruit peels) from the Amazon region for biosurfactant production.

Effects of culture filtrates of endophytic fungi obtained from Piper aduncum L. on the growth of mycobacterium tuberculosis

Substances that inhibit the growth of Mycobacterium tuberculosis could potentially be used as antibiotics. These substances could also be added to test culture media to improve the speed of tuberculosis diagnosis. The aim of this work was to investigate the influence of culture filtrates of endophytic fungi isolated from P. aduncum L. on the growth of M. tuberculosis. To achieve this objective, the following methodology was used: a) endophytic fungi were isolated from the leaves and stems of P. aduncum L.; b) the isolated fungi were submitted to submerged bioprocessing; c) culture filtrates from the bioprocess were assayed to evaluate their effect on the growth of M. tuberculosis. We isolated 315 fungal types, which represented 85 morphologies, from different parts of P. aduncum L. The bioassays were performed on 82 culture filtrates and 6 plant extracts and resulted in the detection of 1 culture filtrate that stimulated the growth of M. tuberculosis and 15 that inhibited microbial growth. None of the phytochemical extracts had an effect on the growth of M. tuberculosis. In conclusion, we observed that the endophytic fungi isolated from P. aduncum L. (Piperaceae) produced extracellular metabolites (present in the culture filtrate) that affect the growth of M. tuberculosis. These compounds have the potential to be used as antimicrobials or in the diagnosis of tuberculosis.

Micoses superficiais na cidade de Manaus, AM, entre março e novembro/2003 / Superficial mycoses in the City of Manaus/AM between March and November/2003

FUNDAMENTOS: Micoses superficiais estritas são infecções fúngicas que se localizam nas camadas superficiais da pele e seus anexos. As micoses superficiais cutâneas representadas pelas dermatofitoses e candidíases podem ultrapassar a camada córnea da pele. Na região amazônica possuem incidência elevada. OBJETIVOS: Estudar as micoses superficiais, estritas e cutâneas, diagnosticadas sob o ponto de vista epidemiológico e micológico. PACIENTES E MÉTODOS: Pacientes com suspeita clínica de micoses superficiais submetidos a exame micológico no período de março a novembro de 2003 no Laboratório de Micologia Médica/CPCS/INPA. RESULTADOS: Foram realizados 394 exames, tendo 256 apresentado diagnóstico positivo. As micoses mais incidentes foram onicomicoses (135) e pitiríase versicolor (98). Malassezia spp. (77) e Candida spp. (72) foram os agentes fúngicos mais isolados. Tinea capitis apresentou maior ocorrência nos pré-escolares (3), e onicomicoses em adultos (94). O sexo feminino foi o mais acometido (91). Todas as classes sociais foram infectadas, com predominância da C (37). CONCLUSÃO: Onicomicoses e pitiríase versicolor acometeram sobretudo adultos. A Tinea capitis ocorre principalmente, em crianças. As micoses superficiais apresentaram mais incidentes nas mulheres. Malassezia spp. e Candida spp. foram os agentes mais isolados.